1V2I
Structure of the hemagglutinin-neuraminidase from human parainfluenza virus type III
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 180 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.500, 93.780, 105.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.300 - 2.200 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.26000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | homology model of hPIV3-HN from NDV-HN PDB entry 1E8T |
RMSD bond length | 0.011 |
RMSD bond angle | 26.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.000 * | 2.280 |
High resolution limit [Å] | 2.200 * | 2.200 |
Rmerge | 0.149 | |
Total number of observations | 227429 * | |
Number of reflections | 39849 * | |
<I/σ(I)> | 10.1 | 1.5 |
Completeness [%] | 94.5 | 96.2 |
Redundancy | 5.7 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 | 298 | PEG 400, potassium phosphate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MES | 50 (mM) | pH6.5 |
2 | 1 | reservoir | 0.15 (M) | ||
3 | 1 | reservoir | PEG400 | 20 (%(w/v)) | |
4 | 1 | drop | protein | 0.8 (mg/ml) |