1V2B
Crystal Structure of PsbP Protein in the Oxygen-Evolving Complex of Photosystem II from Higher Plants
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2001-11-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.035, 1.00907, 1.00720 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.520, 90.442, 52.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.18744 |
| Rwork | 0.186 |
| R-free | 0.20697 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.946 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.600 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.073 | 0.307 |
| Number of reflections | 47044 | |
| <I/σ(I)> | 7.8 | 2.4 |
| Completeness [%] | 98.7 | 97.3 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG4000, MES, lithium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
