1V25

Crystal structure of tt0168 from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-12-11
Detector	RIGAKU RAXIS V
Wavelength(s)	0.9840
Spacegroup name	P 21 21 21
Unit cell lengths	64.520, 101.151, 176.487
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.600 - 2.300
R-factor	0.20807
Rwork	0.204
R-free	0.23956
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.960
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	51688
Completeness [%]	99.3	96.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	MES, ammonium sulfate, PEG-MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K