1V0S

Uninhibited form of Phospholipase D from Streptomyces sp. strain PMF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM1A
Synchrotron site	ESRF
Beamline	BM1A
Temperature [K]	120
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	57.410, 57.370, 68.620
Unit cell angles	90.00, 93.45, 90.00

Refinement procedure

Resolution	69.010 - 1.750
R-factor	0.153
Rwork	0.151
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	BASED ON PDB ENTRY 1F0I
RMSD bond length	0.019
RMSD bond angle	1.761
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.010
High resolution limit [Å]	1.750	1.750
Rmerge	0.080
Number of reflections	39534
<I/σ(I)>	8.4
Completeness [%]	92.1
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.4		0.2 M NH4AC, 0.1 M CITRATE/ PHOSPHATE BUFFER AT PH5.4, 27.5% PEG 4000. CRYSTALS WERE THEREAFTER BACKSOAKED TWICE IN A PHOSPHATE-FREE BUFFER TO REMOVE TRACE-AMOUNTS OF PHOSPHATE IN THE ACTIVE SITE., pH 5.40