1V0M
Xylanase Xyn10a from Streptomyces lividans in complex with xylobio-deoxynojirimycin at pH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.884, 46.081, 85.789 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.650 - 1.070 |
| R-factor | 0.099 |
| Rwork | 0.098 |
| R-free | 0.12100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1od8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.652 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.110 |
| High resolution limit [Å] | 1.070 | 1.070 |
| Rmerge | 0.063 | 0.466 |
| Number of reflections | 117436 | |
| <I/σ(I)> | 25.54 | 2.94 |
| Completeness [%] | 94.4 | 81.2 |
| Redundancy | 5.7 | 5.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM IMIDAZOLE, PH 7.5 16% PEG 5K MME, 5% ISOPROPANOL |
