1V05

Dimerization of human Filamin C: crystal structure of the domain 24

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-15
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	48.099, 48.099, 117.485
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.730 - 1.430
R-factor	0.18771
Rwork	0.186
R-free	0.20498
Structure solution method	OTHER
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.530
High resolution limit [Å]	1.430	1.430
Rmerge	0.037	0.225
Number of reflections	15602
<I/σ(I)>	42	13
Completeness [%]	99.8	100
Redundancy	16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		1.6M NA CITRATE, 0.1M HEPES, PH7.5, pH 7.50