1V04
serum paraoxonase by directed evolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Collection date | 2003-12-15 |
Wavelength(s) | 0.9796, 0.9794 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 98.440, 98.440, 139.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.186 |
Rwork | 0.185 |
R-free | 0.21700 |
Structure solution method | SIRAS |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.086 | 0.660 |
Number of reflections | 33505 | |
<I/σ(I)> | 12.7 | 2.7 |
Completeness [%] | 99.7 | 97.9 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 20% PEG 3350, 0.2M AMMONIUM DIHYDROGEN PHOSPHATE, pH 4.60 |