1UZY
Erythrina crystagalli lectin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2001-12-15 |
Detector | ADSC CCD |
Spacegroup name | P 65 |
Unit cell lengths | 134.018, 134.018, 81.636 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ax1 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | 0.310 |
Number of reflections | 56451 | |
<I/σ(I)> | 18.44 | 6.22 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | RESERVOIR CONTAINED 70% MPD AND 0.1M HEPES, PH 7.0 |