1UZQ

Integrin binding cbEGF22-TB4-cbEGF33 fragment of human fibrillin-1, apo form cbEGF23 domain only.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-12-15
Detector	MARRESEARCH 300MM
Spacegroup name	I 2 2 2
Unit cell lengths	42.400, 70.800, 102.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.254
Rwork	0.254
R-free	0.33000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PBD FIBRILLIN SM BOUND FORM
RMSD bond length	0.012
RMSD bond angle	1.750
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.087	0.344
Number of reflections	6275
<I/σ(I)>	19.4	5.8
Completeness [%]	99.0	98.5
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5		SITTING DROP VAPOUR DIFFUSION, 3 MICROLITRE OF 25MG/ML PROTEIN, 10MM TRIS PH7.5 + 0.5 MICROLITRES OF 40% V/V POLYPROPYLENE GLYCOL P400 + 2.5 MICROLITRES RESERVOIR SOLUTION (0.2M LISULFATE, 0.1M TRIS PH 8.5, 30% PEG 4000) BOTH PROTEIN AND RESERVOIR SOLUTIONS CONTAINED 5MM EDTA