1UZA
Crystallographic structure of a feruloyl esterase from Aspergillus niger
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.298, 39.681, 77.071 |
| Unit cell angles | 75.24, 78.82, 71.30 |
Refinement procedure
| Resolution | 74.540 - 1.500 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tgl |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.766 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.510 |
| Rmerge | 0.060 | 0.170 |
| Number of reflections | 64076 | |
| <I/σ(I)> | 11.5 | 2.5 |
| Completeness [%] | 94.4 | 95.3 |
| Redundancy | 2.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 1.0 M AMMONIUM SULPHATE, 0.1M NA ACETATE PH 4.5 |
