1UZ0

Carbohydrate binding module (CBM6cm-2) from Cellvibrio mixtus lichenase 5A in complex with Glc-4Glc-3Glc-4Glc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Collection date	2003-11-15
Spacegroup name	P 21 21 2
Unit cell lengths	37.924, 102.410, 31.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.300 - 1.900 *
R-factor	0.152
Rwork	0.150
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gmm
RMSD bond length	0.021
RMSD bond angle	1.759
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.300	1.980
High resolution limit [Å]	1.900	1.900
Rmerge	0.112	0.353
Number of reflections	10234
<I/σ(I)>	4.5	2.3
Completeness [%]	99.6	99
Redundancy	4.7	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		11% PEG 6000, 2.0 M NACL, pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	PEG6000	12 (%(w/v))
3	1	reservoir		2.2 (M)