1UYZ

Carbohydrate binding module (CBM6cm-2) from Cellvibrio mixtus lichenase 5A in complex with xylotetraose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Collection date	2003-04-15
Spacegroup name	P 21 21 21
Unit cell lengths	64.010, 67.394, 85.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.700 - 1.600
R-factor	0.162
Rwork	0.161
R-free	0.17800
Structure solution method	OTHER
RMSD bond length	0.008
RMSD bond angle	1.166
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	FFT
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.400	1.670
High resolution limit [Å]	1.600	1.600
Rmerge	0.057	0.238
Number of reflections	49390
<I/σ(I)>	4.5	3
Completeness [%]	100.0	100
Redundancy	4.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		11% PEG 6000, 2.0 M NACL, pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	PEG6000	12 (%(w/v))
3	1	reservoir		2.2 (M)