1UYY

Carbohydrate binding module (CBM6cm-2) from Cellvibrio mixtus lichenase 5A in complex with cellotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Collection date	2003-03-15
Spacegroup name	P 21 21 21
Unit cell lengths	63.550, 67.650, 85.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.700 - 1.470
R-factor	0.175
Rwork	0.174
R-free	0.18900
Structure solution method	OTHER
RMSD bond length	0.008
RMSD bond angle	1.201
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	FFT
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	1.520
High resolution limit [Å]	1.470	1.470
Rmerge	0.035	0.221
Number of reflections	63196
<I/σ(I)>	12.6	3.4
Completeness [%]	99.7	98.4
Redundancy	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		11% PEG 6000, 2.0 M NACL, pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	PEG6000	12 (%(w/v))
3	1	reservoir		2.2 (M)