1UYW
Crystal Structure of the antiflavivirus Fab4g2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2003-07-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.564, 89.611, 138.136 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.540 - 2.000 |
| R-factor | 0.20087 |
| Rwork | 0.199 |
| R-free | 0.21953 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yec |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.278 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.536 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.091 | 0.291 |
| Number of reflections | 60545 | |
| <I/σ(I)> | 5.9 | 1.7 |
| Completeness [%] | 97.0 | 83.9 |
| Redundancy | 4.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 13% PEG8K, 100MM HEPES PH=7.5, 5MM B-OG, pH 7.50 |
