1UYQ
mutated b-glucosidase A from Paenibacillus polymyxa showing increased stability
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 136.416, 136.416, 173.341 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.950 - 2.200 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.22700 |
| Structure solution method | OTHER |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.573 | 2.350 |
| High resolution limit [Å] | 2.100 | 2.210 |
| Rmerge | 0.154 | 0.450 |
| Number of reflections | 47794 | |
| <I/σ(I)> | 4.6 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | DROP: 1 MICROL PROTEIN (8 MG/ML)+ 1 MICROL PB(3M, PH=7)+1 MICROL INH (35MM) RESERVOIR: 1.3M PB, pH 7.00 |
