1UYO
Translocator domain of autotransporter NalP from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Collection date | 2002-06-15 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.430, 84.893, 122.983 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.200 |
| R-factor | 0.219 |
| Rwork | 0.215 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.920 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.150 * | 0.359 * |
| Total number of observations | 45309 * | |
| Number of reflections | 5100 | |
| <I/σ(I)> | 9.4 | 3 |
| Completeness [%] | 98.6 | 94.8 |
| Redundancy | 8.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 28 * | 88 MM TRIS-HCL, PH 7.0, 9% PEG2000MME 18% MPD. PROTEIN WAS CRYSTALLISED IN THE PRESENSE OF 0.06%, N-DECYLPENTAOXYETHYLENE AND 0.5% HEPTYL-B-GLUCOPYRANOSIDE AND 10 MM DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Se-Met NalP~beta | 8.5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 0.06 (%(w/v)) | ||
| 4 | 1 | drop | heptyl-beta-glucopyranoside | 0.5 (%(w/v)) | |
| 5 | 1 | reservoir | Tris-HCl | 88 (mM) | pH7.0 |
| 6 | 1 | reservoir | PEG2000 MME | 9 (%(w/v)) | |
| 7 | 1 | reservoir | MPD | 18 (%(v/v)) |
