1UXB

ADENOVIRUS AD19p FIBRE HEAD in complex with sialyl-lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-08
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	60.700, 68.700, 74.700
Unit cell angles	90.00, 94.90, 90.00

Refinement procedure

Resolution	74.540 - 1.750
R-factor	0.175
Rwork	0.175
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uxa
RMSD bond length	0.016
RMSD bond angle	1.894
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.540	1.840
High resolution limit [Å]	1.750	1.750
Rmerge	0.106	0.280
Number of reflections	62060
<I/σ(I)>	3.2063	2.39
Completeness [%]	100.0	100
Redundancy	4.34	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		RESERVOIR: 24 % PEG8000, 50 MM ZINC ACETATE, 100 MM HEPES. PROTEIN: 30 MM TRIS-HCL, PH 7.5, 150 MM NACL.