1UXA
ADENOVIRUS AD37 FIBRE HEAD in complex with sialyl-lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.700, 68.700, 74.700 |
| Unit cell angles | 90.00, 94.90, 90.00 |
Refinement procedure
| Resolution | 45.180 - 1.500 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qhv |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.066 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.180 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.073 | 0.250 |
| Number of reflections | 87661 | |
| <I/σ(I)> | 5.059 | 2.21 |
| Completeness [%] | 92.3 | 66.6 |
| Redundancy | 3.33 | 2.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | RESERVOIR: 24 % PEG8000, 50 MM ZINC ACETATE, 100 MM HEPES, PROTEIN: 30 MM TRIS-HCL, PH 7.5, 150 MM NACL |
