1UWZ
Bacillus subtilis cytidine deaminase with an Arg56 - Ala substitution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-22 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.743, 66.418, 55.388 |
| Unit cell angles | 90.00, 115.60, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.990 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.21100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1jtk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.114 | 0.388 |
| Number of reflections | 15794 | |
| <I/σ(I)> | 10.8 | 1.3 |
| Completeness [%] | 94.0 | 54 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | VAPOUR DIFFUSION AT RT: PROTEIN: 4.6 MG/ML + 5 MM TETRAHYDRODEOXYURIDINE PRECIPITANT: 17-23 % MPD,10 MM CALCIUM CHLORIDE, 0.1 M SODIUM ACETATE, pH 4.60 |
