1UWN

The Initial Events in the Photocycle of Photoactive Yellow Protein: A Common Mechanism on Light Activation in Photoreceptor Proteins

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	85
Detector technology	CCD
Collection date	2002-03-30
Detector	ADSC CCD
Wavelength(s)	0.9393, 0.9796
Spacegroup name	P 63
Unit cell lengths	65.922, 65.922, 40.584
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 * - 1.200
R-factor	0.125
Rwork	0.124
R-free	0.14300 *
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.830
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.230
High resolution limit [Å]	1.200	1.200
Rmerge	0.033	0.171
Total number of observations	159748 *
Number of reflections	60337 *	4034 *
<I/σ(I)>	15.9	5.5
Completeness [%]	97.1 *	91.1 *
Redundancy	5.2	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	2.25 M NH4SO4, 20 MM SODIUM PHOSPHATE PH 7, VAPOR DIFFUSION, HANGING DROP, TEMP 298K