1UWM

reduced ferredoxin 6 from Rhodobacter capsulatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-15
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	45.542, 50.335, 55.349
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.830 - 2.000
R-factor	0.23
Rwork	0.230
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e9m
RMSD bond length	0.014
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.082	0.273
Number of reflections	9100
<I/σ(I)>	14.29	5.62
Completeness [%]	98.6	96.6
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	288	7.2 M SODIUM FORMATE, IMIDAZOLE PH 7.6, 15 DEG, REDUCTION WITH SODIUM DITHIONITE UNDER 2PPM O2