1UWK
The High Resolution Structure of Urocanate Hydratase from Pseudomonas putida in complex with urocanate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.429, 71.618, 129.158 |
| Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.190 |
| R-factor | 0.1396 |
| R-free | 0.17900 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.033 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.210 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.080 | 0.510 |
| Number of reflections | 1453010 | |
| <I/σ(I)> | 13 | 2 |
| Completeness [%] | 97.0 | 90 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 24% PEG8000, 0.1M SODIUM CACODYLATE (PH 5.8), 0.16M SODIUM ACETATE, 0.1% BETA-MERCAPTOETHANOL |
