1UWI
CRYSTAL STRUCTURE OF MUTATED BETA-GLYCOSIDASE FROM SULFOLOBUS SOLFATARICUS, WORKING AT MODERATE TEMPERATURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.082, 132.425, 219.149 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.440 - 2.550 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gow |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.190 | 0.500 |
| Number of reflections | 75479 | |
| <I/σ(I)> | 3.5 | 1.5 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 8.3 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | PROTEIN WAS CRYSTALLIZED FROM 25% PEG 4K, 0.2 M NACL, PH=9, pH 9.00 |
