1UWA
L290F mutant rubisco from chlamydomonas
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Collection date | 2003-05-03 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.983, 177.709, 122.663 |
| Unit cell angles | 90.00, 117.70, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gk8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.235 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.310 |
| High resolution limit [Å] | 2.300 | 2.260 |
| Rmerge | 0.140 | 0.430 |
| Number of reflections | 264805 | |
| <I/σ(I)> | 10 | 1.6 |
| Completeness [%] | 96.6 | 93.4 |
| Redundancy | 22.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 291 | HEPES PH 7.5, 8-12% PEG 4 50 MM NAHCO3, 5 MM MGCL2, 50 UM 2-CABP, 18 DEG C, 10-15 MG PROTEIN |
