1UW8
CRYSTAL STRUCTURE OF OXALATE DECARBOXYLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-21 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 154.663, 154.663, 122.822 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 91.290 - 2.000 |
| R-factor | 0.129 |
| Rwork | 0.127 |
| R-free | 0.15800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j58 |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 1.409 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.084 | 0.328 |
| Number of reflections | 38069 | |
| <I/σ(I)> | 34.4 | 6.7 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 23.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 18 * | 8% PEG 8000, TRIS-HCL PH8.5, 18 DEG C, pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH7.0 |
| 3 | 1 | reservoir | PEG8000 | 8 (%) | |
| 4 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
