1UW8

CRYSTAL STRUCTURE OF OXALATE DECARBOXYLASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-09-21
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	154.663, 154.663, 122.822
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	91.290 - 2.000
R-factor	0.129
Rwork	0.127
R-free	0.15800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j58
RMSD bond length	0.013 *
RMSD bond angle	1.409 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.084	0.328
Number of reflections	38069
<I/σ(I)>	34.4	6.7
Completeness [%]	100.0	99.8
Redundancy	23.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	18 *	8% PEG 8000, TRIS-HCL PH8.5, 18 DEG C, pH 8.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	drop	Tris	20 (mM)	pH7.0
3	1	reservoir	PEG8000	8 (%)
4	1	reservoir	Tris	100 (mM)	pH8.5