1UVX

Heme-ligand tunneling on group I truncated hemoglobins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-09-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	34.499, 52.932, 66.993
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.450
R-factor	0.216
Rwork	0.210
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dly
RMSD bond length	0.003 *
RMSD bond angle	0.572 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.154	0.432
Number of reflections	4801
<I/σ(I)>	8	1.8
Completeness [%]	98.4	95.4
Redundancy	3.6	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.5	4 *	Pesce, A., (2000) EMBO J., 19, 2424. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	ammonium sulfate	35 (%)
3	1	reservoir	sodium acetate	50 (mM)