1UVT
BOVINE THROMBIN--BM14.1248 COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS-NICOLET X100 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 87.500, 87.500, 103.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.900 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.099 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.7 (M) | |
| 2 | 1 | reservoir | Tris | 0.1 (M) | |
| 3 | 1 | drop | ammonium sulfate | 0.85 (M) | |
| 4 | 1 | drop | Tris | 0.05 (M) | |
| 5 | 1 | drop | protain | 5 (mg/ml) |
