1UVJ

The structural basis for RNA specificity and Ca2 inhibition of an RNA-dependent RNA polymerase phi6p2 with 7nt RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	150
Detector technology	CCD
Collection date	2002-01-15
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	105.062, 93.510, 140.711
Unit cell angles	90.00, 101.19, 90.00

Refinement procedure

Resolution	20.000 * - 1.900
R-factor	0.242
Rwork	0.242
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hhs
RMSD bond length	0.012
RMSD bond angle	22.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.085
Total number of observations	1733793 *
Number of reflections	209896 *
<I/σ(I)>	13.5	1.2
Completeness [%]	99.7	99.8
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.7 *	293 *	Butcher, S.J., (2000) Acta Crystallogr.,Sect.D, D56, 1473. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	PEG8000	10 (%)
3	1	reservoir	MES	0.1 (M)	pH6.7