1UV6
X-ray structure of acetylcholine binding protein (AChBP) in complex with carbamylcholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 140.900, 140.900, 240.412 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.500 |
| Rwork | 0.227 |
| R-free | 0.26400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i9b |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.460 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.123 | 0.675 |
| Number of reflections | 80976 | |
| <I/σ(I)> | 13 | 2.8 |
| Completeness [%] | 96.0 | 98 |
| Redundancy | 12.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | CAPS PH 10.5, AMMONIUM SULFATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 0.1 (M) | pH8.0 |
| 2 | 1 | drop | carbamylcholine | 0.8 (mM) | |
| 3 | 1 | drop | AChBP | 10 (mg/ml) | |
| 4 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 5 | 1 | reservoir | CAPS | 0.1 (M) | pH10.5 |
