1UUY
Structure of a molybdopterin-bound cnx1g domain links molybdenum and copper metabolism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 171.090, 171.090, 171.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.000 - 1.450 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.17900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uux |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.450 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.020 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.055 | 0.400 |
| Number of reflections | 36739 | |
| <I/σ(I)> | 6.2678 | 1.78 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 6.88 | 6.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 3.2 M SODIUM FORMATE ANAEROBIC, pH 7.80 |
