1UUX
Structure of a molybdopterin-bound cnx1g domain links molybdenum and copper metabolism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 171.034, 171.034, 171.034 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eav |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.509 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.880 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.057 | 0.390 |
| Number of reflections | 28748 | |
| <I/σ(I)> | 7.6484 | 1.91 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.78 | 7.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 3.2 M SODIUM FORMATE ANAEROBIC, pH 7.80 |
