1UUR
Structure of an activated Dictyostelium STAT in its DNA-unbound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 121.600, 144.000, 67.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.26900 * |
Structure solution method | MIRAS |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.105 * | 0.351 * |
Number of reflections | 15971 | |
<I/σ(I)> | 14.6 | 4.5 |
Completeness [%] | 95.8 | 75.9 |
Redundancy | 7.4 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 250 (mM) | ||
2 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
3 | 1 | drop | PMSF | 0.2 (mM) | |
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | drop | protein | 6-7 (mg/ml) | |
6 | 1 | reservoir | magnesium acetate | 150 (mM) | |
7 | 1 | reservoir | ammonium acetate | 200 (mM) | |
8 | 1 | reservoir | HEPES | 50 (mM) | pH7. |
9 | 1 | reservoir | PEG4000 | 5 (%) |