1UUQ

Exo-mannosidase from Cellvibrio mixtus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Collection date	2002-11-15
Spacegroup name	P 21 21 2
Unit cell lengths	91.221, 101.759, 50.248
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.880 - 1.500
R-factor	0.123
Rwork	0.121
R-free	0.15200
Structure solution method	OTHER
RMSD bond length	0.010
RMSD bond angle	1.328
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.060	0.267
Number of reflections	73917
<I/σ(I)>	20.8	3.57
Completeness [%]	97.8	81.7
Redundancy	6	3.13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		24% PEG 4000, 0.1M NA ACETATE PH 4.6, 0.2M AMMONIUM SULFATE