1UUH

Hyaluronan binding domain of human CD44

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-15
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	48.881, 77.266, 87.668
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.310 * - 2.200
Rwork	0.186
R-free	0.24000 *
Structure solution method	OTHER
RMSD bond length	0.011 *
RMSD bond angle	1.309 *
Phasing software	SOLVE/RESOLVE
Refinement software	REFMAC (5.1.9999)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.310	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.076	0.181 *
Number of reflections	17476
<I/σ(I)>	7.2989	3.16
Completeness [%]	99.4 *	98.2 *
Redundancy	3.8 *	3.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5		HANGING-DROP PROTEIN 13 MG/ML; WELL BUFFER 12% PEG 3350, 50MM NACL, 10% GLYCEROL, pH 5.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	12 (%)
2	1	reservoir		50 (mM)
3	1	reservoir	glycerol	10 (%)