1UUF
crystal structure of a zinc-type alcohol dehydrogenase-like protein yahK
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2839,1.846,0.9796 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.536, 78.805, 70.685 |
| Unit cell angles | 90.00, 109.60, 90.00 |
Refinement procedure
| Resolution | 19.700 - 1.760 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.20800 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.860 | 1.860 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.077 | 0.178 |
| Number of reflections | 35671 | |
| <I/σ(I)> | 5.6 | 3.4 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.75 | 14% MPD, NACL 0.1M, TRIS 0.1M, SODIUM ACETATE 10MM, ZN ACETATE 0.1MM, PH 4.75 |
