1UU1
Complex of Histidinol-phosphate aminotransferase (HisC) from Thermotoga maritima (Apo-form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.610, 136.519, 92.815 |
Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.380 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h1c |
RMSD bond length | 0.011 |
RMSD bond angle | 1.478 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.450 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.089 * | 0.474 |
Total number of observations | 120299 * | |
Number of reflections | 49556 | |
<I/σ(I)> | 12.7 | 4.22 |
Completeness [%] | 90.6 | 91.3 |
Redundancy | 2.3 * | 2.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 * | 20 * | pH 7.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
2 | 1 | drop | dithiothreitol | 10 (mM) | |
3 | 1 | drop | EDTA | 2 (mM) | |
4 | 1 | drop | protein | 35 (mg/ml) | |
5 | 1 | reservoir | ethylene glycol | 50 (%(v/v)) | |
6 | 1 | reservoir | PEG1000 | 5 (%(w/v)) | pH5.1 |
7 | 1 | reservoir | sodium acetate |