1UTU

Crystal structure of novel protein EMSY truncate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-10-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	51.178, 32.642, 51.607
Unit cell angles	90.00, 92.51, 90.00

Refinement procedure

Resolution	27.580 - 2.000
R-factor	0.207
Rwork	0.207
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.700
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.600	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.065	0.240
Number of reflections	9491
<I/σ(I)>	10.5	3.1
Completeness [%]	92.9	88.2
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.2M NACL, 20% PEG 3350, PH 6.9