1UTC

Clathrin terminal domain complexed with TLPWDLWTT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	61.301, 61.301, 420.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.900 * - 2.300
R-factor	0.197
Rwork	0.194
R-free	0.25000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c9i
RMSD bond length	0.016
RMSD bond angle	1.600 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.9999)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.900 *	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.076 *	0.329 *
Total number of observations	495661 *
Number of reflections	37078 *
<I/σ(I)>	9.3131	2.02
Completeness [%]	99.4	97.2 *
Redundancy	5.8 *	4.33

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9		VAPOUR DIFFUSION OVER A RESERVOIR CONTAINING 20% PEG8000, 200 MM MGCL2, 4 MM DTT,100 MM CAPS PH 9, DRIED UP DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20 (%(w/v))
2	1	reservoir		200 (mM)
3	1	reservoir	dithiothreitol	4 (mM)
4	1	reservoir	CAPS	100 (mM)	pH9.