1UT1

DraE adhesin from Escherichia Coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-15
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	68.879, 108.507, 119.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.175
Rwork	0.173
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1usz
RMSD bond length	0.012
RMSD bond angle	1.044
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.000	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.048	0.252
Number of reflections	94785
<I/σ(I)>	9.6	3
Completeness [%]	95.7	77.2
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		2.0 M AMMONIUM SULPHATE, 0.1M TRIS-HCL, PH 7.0