1USZ
SeMet AfaE-3 adhesin from Escherichia Coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-15 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 141.300, 141.300, 141.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.280 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.667 |
| Data scaling software | SCALA |
| Phasing software | RANTAN |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.084 | 0.320 |
| Number of reflections | 5108 | |
| <I/σ(I)> | 7.8 | 1.7 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.0 M AMMONIUM SULPHATE, 2% PEG 400, 0.1M NAHEPES PH 6.8 |
