1USP

Organic Hydroperoxide Resistance Protein from Deinococcus radiodurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	45.408, 56.600, 49.458
Unit cell angles	90.00, 90.27, 90.00

Refinement procedure

Resolution	49.400 - 1.900
R-factor	0.173
Rwork	0.171
R-free	0.20400
Structure solution method	SAD
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXD
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.054	0.309
Number of reflections	18043
<I/σ(I)>	8.5	2.4
Completeness [%]	91.0	62.5
Redundancy	3.1	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.2		pH 7.20