1USK
L-leucine-binding protein with leucine bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Collection date | 2003-03-15 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.421, 70.140, 79.902 |
| Unit cell angles | 112.79, 93.23, 90.97 |
Refinement procedure
| Resolution | 38.000 * - 2.000 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.25300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1usi |
| RMSD bond length | 0.022 * |
| RMSD bond angle | 1.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.164 | 0.431 |
| Number of reflections | 72985 | |
| <I/σ(I)> | 4.6 | 1.4 |
| Completeness [%] | 79.1 | 22.7 |
| Redundancy | 2.8 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 4 * | PEG 4000, SODIUM CITRATE, PH 5.6, 2-PROPANOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 3 | 1 | reservoir | PEG5000 | 20 (%) | |
| 4 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
| 5 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 6 | 1 | reservoir | 10 (mM) |
