1USG
L-leucine-binding protein, apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Collection date | 2003-03-15 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.410, 79.200, 65.180 |
Unit cell angles | 90.00, 133.60, 90.00 |
Refinement procedure
Resolution | 32.600 * - 1.530 |
R-factor | 0.191 |
Rwork | 0.189 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2lbp |
RMSD bond length | 0.015 |
RMSD bond angle | 1.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.620 * | 1.560 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.046 | 0.177 |
Number of reflections | 48509 | |
<I/σ(I)> | 25.4 | 4.9 |
Completeness [%] | 95.7 | 93.3 |
Redundancy | 3.2 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | 4 * | PEG 5000, SODIUM ACETATE PH 4.6, AMMONIUM SULPHATE, COCL2. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
3 | 1 | reservoir | PEG5000 | 20 (%) | |
4 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
5 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
6 | 1 | reservoir | 10 (mM) |