1URP
D-RIBOSE-BINDING PROTEIN FROM ESCHERICHIA COLI
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 1996-06 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.099, 120.954, 64.131 |
Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
Rwork | 0.235 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | FOR LIGAND-FREE MUTANT RBP MOLECULE B. |
RMSD bond length | 0.005 |
RMSD bond angle | 1.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.056 | 0.172 |
Number of reflections | 36827 | |
<I/σ(I)> | 9.8 | 4.6 |
Completeness [%] | 90.7 | 79.1 |
Redundancy | 2 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5 | CRYSTALS WERE OBTAINED FROM SOLUTIONS OF 7.5 - 15 MG/ML RBP, 21% PEG4000, 50-100 MM SODIUM CITRATE, PH 4, 5% GLYCEROL, BY HANGING DROP VAPOUR DIFFUSION., pH 5.0, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.5-15 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 21 (%) | |
3 | 1 | reservoir | sodium citrate | 50-100 (mM) | |
4 | 1 | reservoir | glycerol | 5 (%) |