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N-TERMINAL DOMAIN OF SIALOADHESIN (MOUSE) IN COMPLEX WITH GLYCOPEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	42.230, 78.460, 34.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.740 - 2.400
R-factor	0.225
Rwork	0.225
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qfo
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.700	2.520
High resolution limit [Å]	2.400	2.400
Rmerge	0.081	0.473
Number of reflections	4194
<I/σ(I)>	7.5	1.6
Completeness [%]	87.7	73.4
Redundancy	3.8	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		30% PEG4000, 0.1 M NA-CITRATE, 0.2 M AMSO4 PH 5.6