1URA

ALKALINE PHOSPHATASE (D51ZN)

Experimental procedure

Collection date	1994-06-29
Spacegroup name	I 2 2 2
Unit cell lengths	194.810, 166.870, 76.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.040
R-factor	0.203
Rwork	0.203
R-free	0.23400
RMSD bond length	0.020
RMSD bond angle	23.600 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.040 *
Rmerge	0.097 *
Total number of observations	367482 *
Number of reflections	73906
Completeness [%]	93.3
Redundancy	4.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9.5 *		Chen, L. C., (1992) Protein Eng., 5, 605. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		20 (%(w/v))
2	1	drop	Tris	100 (mM)
3	1	drop		10 (mM)
4	1	drop		0.01 (mM)
5	1	drop	protain	30 (mg/ml)
6	1	reservoir		31.6 (%(w/v))
7	1	reservoir	Tris	100 (mM)
8	1	reservoir		10 (mM)