1UR3
Crystal structure of the apo form of the E.coli ydhF protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2002-03-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97917,0.98735,0.96 |
| Spacegroup name | P 63 |
| Unit cell lengths | 87.722, 87.722, 66.168 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.080 - 2.570 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.25600 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.000 | 2.660 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.078 | 0.322 |
| Number of reflections | 9276 | |
| <I/σ(I)> | 6.3 | 1.7 |
| Completeness [%] | 98.7 | 98.7 |
| Redundancy | 5.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PEG 4000 17.5%, IMIDAZOLE/MALATE 0.2M PH 7.0, AMMONIUM SULFATE 0.1M, GLYCEROL 2.5%, SPERMIDINE 0.1M |
