1UQY
Xylanase Xyn10B mutant (E262S) from Cellvibrio mixtus in complex with xylopentaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.158, 67.901, 104.794 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.720 |
| R-factor | 0.142 |
| Rwork | 0.140 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1clx |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.539 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.780 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.038 | 0.174 |
| Number of reflections | 34539 | |
| <I/σ(I)> | 46 | 10.35 |
| Completeness [%] | 99.6 | 98.7 |
| Redundancy | 6.02 | 5.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 18 * | 0.2M MGCL2 0.1M TRIS HCL PH8, 30% PEG 4K, pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.20 (M) | ||
| 2 | 1 | reservoir | Tris | 0.1 (M) | pH8.0 |
| 3 | 1 | reservoir | PEG4000 | 30 (%) | |
| 4 | 1 | drop | protein | 25 (mg/ml) | |
| 5 | 1 | drop | xylooligosaccharide | 10 (mM) |
