1UQT
Trehalose-6-phosphate from E. coli bound with UDP-2-fluoro glucose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.919, 101.521, 118.207 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.000 |
| R-factor | 0.22 |
| Rwork | 0.219 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gz5 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.558 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.061 | 0.455 |
| Number of reflections | 67542 | |
| <I/σ(I)> | 18.7 | 2.33 |
| Completeness [%] | 97.8 | 83.8 |
| Redundancy | 3.7 | 2.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 30% PEG 4000, 200MM AMMONIUM ACETATE, 100MM TRISHCL PH 8 |
