1UP1

UP1, THE TWO RNA-RECOGNITION MOTIF DOMAIN OF HNRNP A1

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1996-04
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	37.990, 43.940, 55.750
Unit cell angles	90.00, 94.20, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.197
Rwork	0.197
R-free	0.25400
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT WITH ANOMALOUS SCATTERING
RMSD bond length	0.012
RMSD bond angle	25.230 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.900
Rmerge	0.062
Total number of observations	123655 *
Number of reflections	14637
<I/σ(I)>	23.6
Completeness [%]	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		25% PEG 4000, 20% MPD OR 10-15% GLYCEROL, 100MM TRIS, PH 8.5